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cloning gene specific guide rna sequences  (Addgene inc)


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    Structured Review

    Addgene inc cloning gene specific guide rna sequences
    Cloning Gene Specific Guide Rna Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna-2+sequence/gRNA+Cloning+Vector+Bbs+I+ver%2E+2+(Plasmid+%2385586)/pmc11494886-326-6-16
    Average 93 stars, based on 10 article reviews
    cloning gene specific guide rna sequences - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Evaluation of a Novel Oncolytic Adenovirus Silencing SYVN1
    Article Snippet: .. A549/PG13-Luc.cl9 cells were co-transfected with pX330-Delta_Puro [ ] in which single guide RNA sequence 5′-CCCCGGACGATATTGAACAA TGG-3′ targeting nt 137–153 of the human TP53 open reading frame was inserted, as described by Ran et al. [ ], along with pX330-U6-Chimeric_BB-CBh-hSpCas9 ([ ]; a gift from Feng Zhang; Addgene plasmid # 42230), using Lipofectamine 3000 (Invitrogen, Bleiswijk, The Netherlands), in Opti-MEM reduced serum medium (Gibco, Bleiswijk, The Netherlands). .. Transiently transfected cells were selected for 2 days in 1 μg/mL puromycin (Sigma-Aldrich, Zwijndrecht, The Netherlands), following which cells were cloned by single-cell plating.

    Article Title: Translational control through differential ribosome pausing during amino acid limitation in mammalian cells
    Article Snippet: pADHS8: pU6-GCN2-2-Cas9-2A-GFP , Cloned from Ran et al., 2013 , Cloned GCN2 (EIF2AK4) guide RNA sequence 3 (Doench et al. 2016, Addgene #75877) into pSpCas9(BB)-2A-GFP (PX458). .. pADHS9: pU6-EEF2K-1-Cas9-2A-BFP , Cloned from Chu et al., 2015 , Cloned EEF2K guide RNA sequence 2 (Doench et al.2016, Addgene #77855) into pU6-(BbsI)_CBh-Cas9-T2A-BFP. .. pADHS10: pU6-EEF2K-2-Cas9-2A-GFP , Cloned from Ran et al., 2013 , Cloned EEF2K guide RNA sequence 3 (Doench et al.2016, Addgene #77856) into pU6-EEF2K-2-Cas9-2A-GFP.

    Article Title: An aggregon in conductin/axin2 regulates Wnt/β-catenin signaling and holds potential for cancer therapy
    Article Snippet: .. In short, a top scoring guide RNA according to the CRISPR Design tool provided by the Zhang Lab (crispr.mit.edu) targeting exon 2 of human AXIN2 (guide RNA sequence: CGAGATCCAGTCGGTGATGG; score: 84) was cloned in the PX458 Cas9/GFP/guideRNA-expression vector purchased from Addgene (#48138). ..

    Article Title: Translational control through differential ribosome pausing during amino acid limitation in mammalian cells
    Article Snippet: pADHS6: pSpCas9(BB)-2A-GFP (PX458) , Ran et al., 2013 , Addgene #48138. .. pADHS7: pU6-GCN2-1-Cas9-2A-BFP , Cloned from Chu et al., 2015 , Cloned GCN2 (EIF2AK4) guide RNA sequence 2 (Doench et al.2016, Addgene #75876) into pU6-(BbsI)_CBh-Cas9-T2A-BFP. .. pADHS8: pU6-GCN2-2-Cas9-2A-GFP , Cloned from Ran et al., 2013 , Cloned GCN2 (EIF2AK4) guide RNA sequence 3 (Doench et al. 2016, Addgene #75877) into pSpCas9(BB)-2A-GFP (PX458).

    Plasmid Preparation:

    Article Title: Evaluation of a Novel Oncolytic Adenovirus Silencing SYVN1
    Article Snippet: .. A549/PG13-Luc.cl9 cells were co-transfected with pX330-Delta_Puro [ ] in which single guide RNA sequence 5′-CCCCGGACGATATTGAACAA TGG-3′ targeting nt 137–153 of the human TP53 open reading frame was inserted, as described by Ran et al. [ ], along with pX330-U6-Chimeric_BB-CBh-hSpCas9 ([ ]; a gift from Feng Zhang; Addgene plasmid # 42230), using Lipofectamine 3000 (Invitrogen, Bleiswijk, The Netherlands), in Opti-MEM reduced serum medium (Gibco, Bleiswijk, The Netherlands). .. Transiently transfected cells were selected for 2 days in 1 μg/mL puromycin (Sigma-Aldrich, Zwijndrecht, The Netherlands), following which cells were cloned by single-cell plating.

    Article Title: KBG syndrome-associated protein ANKRD11 regulates SETD5 expression to modulate rRNA levels and translation
    Article Snippet: pSpCas9(BB)-2A-puro , Addgene , Addgene Plasmid #48139. .. pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3) , This paper , Addgene Plasmid #236608. .. pSpCas9(BB)-2A-puro-mAnkrd11 (intron 4) , This paper , Addgene Plasmid #236609.

    CRISPR:

    Article Title: Discovery and design of molecular glue enhancers of CDK12–DDB1 interactions for targeted degradation of cyclin K
    Article Snippet: .. CRISPR knockout plasmids were created by cloning gene-specific guide RNA sequences into the lentiCRISPR v2 vector (Addgene #52961). .. HEK293T cells were transduced with the CRISPR knockout plasmids and packaging plasmids (MISSION system, Sigma Aldrich) using FuGENE HD (Promega) according to the manufacturer's instructions.

    Article Title: An aggregon in conductin/axin2 regulates Wnt/β-catenin signaling and holds potential for cancer therapy
    Article Snippet: .. In short, a top scoring guide RNA according to the CRISPR Design tool provided by the Zhang Lab (crispr.mit.edu) targeting exon 2 of human AXIN2 (guide RNA sequence: CGAGATCCAGTCGGTGATGG; score: 84) was cloned in the PX458 Cas9/GFP/guideRNA-expression vector purchased from Addgene (#48138). ..

    Knock-Out:

    Article Title: Discovery and design of molecular glue enhancers of CDK12–DDB1 interactions for targeted degradation of cyclin K
    Article Snippet: .. CRISPR knockout plasmids were created by cloning gene-specific guide RNA sequences into the lentiCRISPR v2 vector (Addgene #52961). .. HEK293T cells were transduced with the CRISPR knockout plasmids and packaging plasmids (MISSION system, Sigma Aldrich) using FuGENE HD (Promega) according to the manufacturer's instructions.

    Cloning:

    Article Title: Discovery and design of molecular glue enhancers of CDK12–DDB1 interactions for targeted degradation of cyclin K
    Article Snippet: .. CRISPR knockout plasmids were created by cloning gene-specific guide RNA sequences into the lentiCRISPR v2 vector (Addgene #52961). .. HEK293T cells were transduced with the CRISPR knockout plasmids and packaging plasmids (MISSION system, Sigma Aldrich) using FuGENE HD (Promega) according to the manufacturer's instructions.

    Clone Assay:

    Article Title: Translational control through differential ribosome pausing during amino acid limitation in mammalian cells
    Article Snippet: pADHS8: pU6-GCN2-2-Cas9-2A-GFP , Cloned from Ran et al., 2013 , Cloned GCN2 (EIF2AK4) guide RNA sequence 3 (Doench et al. 2016, Addgene #75877) into pSpCas9(BB)-2A-GFP (PX458). .. pADHS9: pU6-EEF2K-1-Cas9-2A-BFP , Cloned from Chu et al., 2015 , Cloned EEF2K guide RNA sequence 2 (Doench et al.2016, Addgene #77855) into pU6-(BbsI)_CBh-Cas9-T2A-BFP. .. pADHS10: pU6-EEF2K-2-Cas9-2A-GFP , Cloned from Ran et al., 2013 , Cloned EEF2K guide RNA sequence 3 (Doench et al.2016, Addgene #77856) into pU6-EEF2K-2-Cas9-2A-GFP.

    Article Title: An aggregon in conductin/axin2 regulates Wnt/β-catenin signaling and holds potential for cancer therapy
    Article Snippet: .. In short, a top scoring guide RNA according to the CRISPR Design tool provided by the Zhang Lab (crispr.mit.edu) targeting exon 2 of human AXIN2 (guide RNA sequence: CGAGATCCAGTCGGTGATGG; score: 84) was cloned in the PX458 Cas9/GFP/guideRNA-expression vector purchased from Addgene (#48138). ..

    Article Title: Translational control through differential ribosome pausing during amino acid limitation in mammalian cells
    Article Snippet: pADHS6: pSpCas9(BB)-2A-GFP (PX458) , Ran et al., 2013 , Addgene #48138. .. pADHS7: pU6-GCN2-1-Cas9-2A-BFP , Cloned from Chu et al., 2015 , Cloned GCN2 (EIF2AK4) guide RNA sequence 2 (Doench et al.2016, Addgene #75876) into pU6-(BbsI)_CBh-Cas9-T2A-BFP. .. pADHS8: pU6-GCN2-2-Cas9-2A-GFP , Cloned from Ran et al., 2013 , Cloned GCN2 (EIF2AK4) guide RNA sequence 3 (Doench et al. 2016, Addgene #75877) into pSpCas9(BB)-2A-GFP (PX458).

    Article Title: Selective use of primate CD4 receptors by HIV-1.
    Article Snippet: .. The D1/D2 domain (nucleotide positions 75–603, minus signal peptide) was amplified by PCR and cloned into pHL-sec [119] (Addgene #99845), which has been optimized for protein production in mammalian cells. .. 13 x 106 293T cells (three 15-cm dishes) were transfected with 25 μg of sCD4 expression plasmids using TransIT-293 (Mirus), and cell supernatant containing secreted sCD4 was harvested at days three and six post transfection.

    other:

    Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
    Article Snippet: This work pKK209 Constitutive expression of BFP-targeting gRNA (gRNA #2) (spacer sequence; GCACATGAAGCTGTACATGG). oKK531 and oKK532 were inserted to pCRISPRia-v2 (addgene #848322) in the same way as pKK89.

    Amplification:

    Article Title: Selective use of primate CD4 receptors by HIV-1.
    Article Snippet: .. The D1/D2 domain (nucleotide positions 75–603, minus signal peptide) was amplified by PCR and cloned into pHL-sec [119] (Addgene #99845), which has been optimized for protein production in mammalian cells. .. 13 x 106 293T cells (three 15-cm dishes) were transfected with 25 μg of sCD4 expression plasmids using TransIT-293 (Mirus), and cell supernatant containing secreted sCD4 was harvested at days three and six post transfection.

    Polymerase Chain Reaction:

    Article Title: Selective use of primate CD4 receptors by HIV-1.
    Article Snippet: .. The D1/D2 domain (nucleotide positions 75–603, minus signal peptide) was amplified by PCR and cloned into pHL-sec [119] (Addgene #99845), which has been optimized for protein production in mammalian cells. .. 13 x 106 293T cells (three 15-cm dishes) were transfected with 25 μg of sCD4 expression plasmids using TransIT-293 (Mirus), and cell supernatant containing secreted sCD4 was harvested at days three and six post transfection.



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    Image Search Results


    Journal: iScience

    Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma

    doi: 10.1016/j.isci.2023.108353

    Figure Lengend Snippet:

    Article Snippet: gRNA sequence 2: AUGUCACCUCUCCUCCACCA , Synthego , N/A.

    Techniques: Recombinant, Lactate Dehydrogenase Assay, Cell Based Assay, Gene Knockout, Enzyme-linked Immunosorbent Assay, shRNA, Sequencing, Software, Gene Expression

    Plasmids used in this work

    Journal: Microbial Cell Factories

    Article Title: Development of an industrial yeast strain for efficient production of 2,3-butanediol

    doi: 10.1186/s12934-022-01924-z

    Figure Lengend Snippet: Plasmids used in this work

    Article Snippet: P58-PDC1 , P58 backbone with 2 gRNA targeting sequence for PDC1 (AGCATCCAACAATTTTTGCA and GATAAGCTTTATGAAGTCAA) , MCB, KU Leuven.

    Techniques: Marker, Plasmid Preparation, Sequencing, Expressing